OBJECTIVE: To evaluate the effects of the petroleum ether extract of Cissus quadrangularis on
the proliferation rate of bone marrow mesenchymal stem cells, the differentiation of marrow
mesenchymal stem cells into osteoblasts (osteoblastogenesis) and extracellular matrix
calcification. This study also aimed to determine the additive effect of osteogenic media and
Cissus quadrangularis on proliferation, differentiation and calcification.
METHODS: MSCs were cultured in media with or without Cissus quadrangularis for 4 weeks and
were then stained for alkaline phosphatase. Extracellular matrix calcification was confirmed by
Von Kossa staining. marrow mesenchymal stem cells cultures in control media and osteogenic
media supplemented with Cissus quadrangularis extract (100, 200, 300 μg/mL) were also
subjected to a cell proliferation assay (MTT).
RESULTS: Treatment with 100, 200 or 300 μg/mL petroleum ether extract of Cissus
quadrangularis enhanced the differentiation of marrow mesenchymal stem cells into ALP-positive
osteoblasts and increased extracellular matrix calcification. Treatment with 300 μg/mL petroleum
ether extract of Cissus quadrangularis also enhanced the proliferation rate of the marrow
mesenchymal stem cells. Cells grown in osteogenic media containing Cissus quadrangularis
exhibited higher proliferation, differentiation and calcification rates than did control cells.
CONCLUSION: The results suggest that Cissus quadrangularis stimulates osteoblastogenesis
and can be used as preventive/alternative natural medicine for bone diseases such as
Fixtro Forte from Haldane Pharma is effective in promoting bone health and fracture healing. It
contains all natural ingredients, the key ingredient is the herbal extract Cissus Quadrangularis
which has undergone extensive research for its effect on bone strengthening, bone density and on
quick healing of fractures. Cissus quadrangularis has profound bone healing properties besides
containing unique nutrients that promote bone formation. Fixtro Forte also contains other key
minerals & vitamins which enhances bone health and supports body’s natural bone building
process while healing from a fracture or surgery.